Great Ships Initiative

 


 


GSI STANDARD OPERATING PROTOCOLS

GSI uses standard operating procedures (SOPs) to implement all test activities.This facilitates consistent conformance to technical and quality system requirements and increases data quality. GSI's SOPs include both programmatic and technical processes and procedures such as sample collection, labeling, analysis and custody; and safety. The SOPs follow a common format and include specific QAQC procedures and metrics. GSI SOPs are grounded in published standard methods. They are also consistent with international and domestic guidelines where they exist. All GSI SOPs are subject to periodic review and revision to assure that the most up to date approaches are employed.

To date approximately 50 GSI SOPs have been developed and finalized, with many more in draft form or planned. Finalized SOPs are listed below by type/scale and include general SOPsbench-scale SOPs, land-based SOPs, shipboard SOPs and multi-scale SOPs (i.e., those that are applicable to multiple scales of testing). Copies of GSI SOPs are free and available by request..


General GSI Standard Operating Procedures (SOPs)

Copies of GSI general SOPs are free and available by request.

Category
Subcategory
Title
Administration
Record Keeping
Procedure for Record Keeping (GSI/SOP/G/A/RK/1)
Testing and Research Activities
Data Management
Procedure for GSI Zooplankton Database Data Entry, Data Quality Control and Database Management (GSI/SOP/G/RA/DM/1)
Procedure for General Data Entry Using Microsoft ® Excel (GSI/SOP/G/RA/DM/2)
Procedure for GSI Protist Database Data Entry, Data Quality Control, and Database Management (GSI/SOP/G/RA/DM/3)
Sample Custody
Procedure for Custody of GSI Samples (GSI/SOP/G/RA/SC/1)
Procedure for Creating Datasheets and Labeling Samples Collected During Bench-Scale Testing (GSI/SOP/G/RA/SC/2)
Procedure for Labeling Sample Collection Containers at the GSI Land-Based RDTE Facility (GSI/SOP/G/RA/SC/3)

 


Bench-Scale GSI Standard Operating Procedures (SOPs)

Copies of GSI bench-scale SOPs are free and available by request.

Category
Subcategory
Title
Testing and Research Activities
General Laboratory
Procedure for Verification of Laboratory Balances (GSI/SOP/BS/RA/GL/1)
Chemical Degradation
Bench-Scale Procedure for Examining the Aquatic Degradation of Active Substance(s) in a Ballast Water Management System (GSI/SOP/BS/RA/CD/1)
Dose Effectiveness
Bench-Scale Procedure for Assessing Dose Effectiveness of a Ballast Water Management System Using a Copepod (GSI/SOP/BS/RA/EF/1)
Bench-Scale Procedure for Assessing Dose Effectiveness of a Ballast Water Management System Using a Cladoceran (GSI/SOP/BS/RA/EF/2)

Procedure for Assessing Dose Effectiveness of a Ballast Water Management System Using Selenastrum capricornutum(GSI/SOP/BS/RA/EF/5)

Procedure for Exposing Test Organisms to a Ballast Water Management System Using Large Volume Influent and Effluent Tanks (GSI/SOP/BS/RA/EF/6)
Residual Toxicity
Procedure for Assessing Chronic Residual Toxicity of a Ballast Treatment System to Ceriodaphia dubia (GSI/SOP/BS/RA/WET/1)
Procedure for Assessing Chronic Residual Toxicity of a Ballast Water Management System to the Fathead Minnow (Pimephales promelas) (GSI/SOP/BS/RA/WET/2)
Procedure for Assessing Chronic Residual Toxicity of a Ballast Water Management System to the Green Alga (Selenastrum capricornutum) (GSI/SOP/BS/RA/WET/3)
Microbial Analysis
Procedure For Conducting Heterotrophic Plate Counts (HPCs) Using IDEXX’s SimPlate® for HPC Method (GSI/SOP/BS/RA/MA/1)
Procedure for the Detection and Enumeration of Enterococcus using Enterolert™ (GSI/SOP/BS/RA/MA/3)
Procedure for the Detection and Enumeration of Total Coliforms and E. coli Using IDEXX's Colilert® (GSI/SOP/BS/RA/MA/4)
Procedure for the Detection and Enumeration of Male-Specific (F+) Coliphage Using Double Agar Layer Technique (DAL) (GSI/SOP/BS/RA/MA/5)
Procedure For Colony Blot Preparation for the Enumeration of Culturable  Vibrio cholerae and Presence of ctxA Gene (GSI/SOP/BS/RA/MA/6)
Procedure for Enumerating Heterotrophic Plate Counts (HPC) Using the Spread Plate Method (GSI/SOP/BS/RA/MA/7)
General Microbiology Preparation Procedures (GSI/SOP/BS/RA/MP/1)
Chemistry
Procedure for Analyzing the Concentration of Ozone in Water (GSI/SOP/BS/RA/C/1)
Procedure for Determining Total Residual Oxidants (TRO) in Water (GSI/SOP/BS/RA/C/2)
Procedures for Measuring Organic Carbon in Aqueous Samples (GSI/SOP/BS/RA/C/3)
Procedure for Determining Percent Transmittance (%T) of Light in Water at 254 nm (GSI/SOP/BS/RA/C/4)
Procedure for Measuring Organic Compounds using High Performance Liquid Chromatography (HPLC) (GSI/SOP/BS/RA/C/5)
Procedure for Analyzing Total Residual Chlorine Concentrations in Water (GSI/SOP/BS/RA/C/6)
Procedure for Analyzing Hydrogen Peroxide Concentrations in Water (GSI/SOP/BS/RA/C/7)
Procedure for Analyzing Total Suspended Solids (TSS), Particulate Organic Matter (POM) and Mineral Matter (MM) (GSI/SOP/BS/RA/C/8)
Procedure for pH Meter Calibration and pH Measurement (GSI/SOP/BS/RA/C/9)
Procedure for Determination of Oxidation-Reduction Potential (ORP) (GSI/SOP/BS/RA/C/11)

 


Land-Based GSI Standard Operating Procedures (SOPs)

Copies of GSI land-based SOPs are free and available by request.

Category
Subcategory
Title
General
Operation
Procedure for Sampling and Analyzing Treated Water in the Retention Tanks Prior to Discharge (GSI/SOP/LB/G/O/2)
Procedure for Cleaning and Verifying Cleanliness of the Retention Tanks and Piping at the GSI Land-Based RDTE Facility (GSI/SOP/LB/G/O/3)
Procedure for Cleaning Sampling Equipment at the GSI Land-Based RDTE Facility (GSI/SOP/LB/G/O/4)
Procedure for Injecting Solids into the GSI Land-Based RDTE Facility (GSI/SOP/LB/G/O/5)
Procedure for Maintaining Solids Suspension in the GSI Land-Based RDTE Facility’s Retention Tanks (GSI/SOP/LB/G/O/7)
Calibration
Calibration and Maintenance of the In-Line ph/Temperature Sensor at the GSI Land-Based RDTE Facility (GSI/SOP/LB/G/C/7)
Calibration of the In-Line Chlorophyll a Sensor at the GSI Land-Based RDTE Facility (GSI/SOP/LB/G/C/8)
Safety
Procedure for Ensuring Worker Health and Safety at the GSI Land-Based RDTE Facility (GSI/SOP/LB/G/S/1)
Testing and Research Activities
Sample Collection
Procedure for Collecting Intake and Discharge Water in Sample Collection Tubs via In-Line Sample Ports (GSI/SOP/LB/RA/SC/1)
Procedure for Collecting Water Chemistry Samples and Data at the GSI Land-Based RDTE Facility (GSI/SOP/LB/RA/SC/2)
Procedure for Protist Sample Collection (GSI/SOP/LB/RA/SC/3)
Procedure for Microbial Sample Collection (GSI/SOP/LB/RA/SC/4)
Procedure for Zooplankton Sample Collection (GSI/SOP/LB/RA/SC/6)
Sample Analysis Procedure for Protist Sample Analysis (GSI/SOP/LB/RA/SA/1)
Procedure for Zooplankton Sample Analysis (GSI/SOP/LB/RA/SA/2))

 


Shipboard GSI Standard Operating Procedures (SOPs)

Copies of GSI shipboard SOPs are free and available by request.

Category
Subcategory
Title
Testing and Research Activities
Operation
Set Up and Operation of the Great Ships Initiative (GSI) Shipboard Sampling System (SSS) (GSI/SOP/SB/O/1)
Sample Collection
Sample Collection Procedures for the Great Ships Initiative (GSI) Shipboard Sampling System (SSS) (GSI/SOP/SB/SC/1 )
Operation and Maintenance of the YSI Pro30 (GSI/SOP/SB/SC/2)
Operation and Maintenance of the AquaFluor® Handheld Fluorometer and Turbidimeter (GSI/SOP/SB/SC/3)
Analysis
Procedure for Shipboard Zooplankton Sample Analysis (GSI/SOP/SB/RA/SA/1)
Procedure for Site Validation and Shipboard Protist Sample Analysis (GSI/SOP/SB/RA/SA/2)
Procedure for the Detection and Enumeration of E. coli Using IDEXX's Colilert-18® (GSI/SOP/SB/RA/SA/4)
Procedure for the Detection and Enumeration of Enterococcus using Enterolert™ (GSI/SOP/SB/RA/SA/5)
Procedure for the Detection of Vibrio cholerae in Ballast Water (GSI/SOP/SB/RA/SA/6)
Procedure for Determining Total Residual Oxidants (TRO) in Water using the HACH Pocket Colorimeter™ II (GSI/SOP/SB/RA/SA/7)

 


Multi-Scale GSI Standard Operating Procedures (SOPs)

Copies of GSI multi-scale SOPs are free and available by request.

Category
Subcategory
Title
Testing and Research Activities
Calibration
Procedure for Calibration, Deployment, and Storage of YSI Multiparameter Water Quality Sondes (GSI/SOP/LB/G/C/4)

 



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